We are a constellation of young scientists at Uppsala University interested in bioimaging with X-ray lasers.
We have been part several seminal experiments which created the field and are now looking to fill several open positions to create the team thatf can take the next step!Open Positions Check our Research
By using high-repetition rate X-ray free-electron lasers we plan to obtain hundreds of millions of diffraction patterns from proteins and macromolecular complexes in different conformations, which we will then classify and reconstruct. To achieve this goal we will develop the science and technology to handle the torrent of data from new X-ray lasers, and create new algorithms to detect structural heterogeneity directly from the diffraction patterns.
Proteins are dynamic structures that fluctuate and it is these transitions that give the protein its function. Given the ultra-short pulse length of free-electron lasers, collected data could be used to study protein dynamics at a very high time resolution. This does however pose great challenges in terms of data analysis to be able to sort through hundreds of thousands of diffraction patterns and from there determine a three-dimensional structure. This challenge will be the focus of this PhD project. This is a truly interdisciplinary research area that brings together physics, biology and data science.
The project, “Serial Femtosecond Crystallography – from nanocrystals to nanoplasma in a flash”, will study the diffraction before destruction of protein crystals with X-ray lasers, using theoretical models and/or experimental measurements.
Sample delivery is critical to the success of any experiment. The project will focus on improving the reliability and size range of electrospray sample delivery for X-ray diffraction imaging experiments. Such developments will pave the way to the analysis of from single proteins data using established 3D reconstruction methods.Click to Apply